Gut microbiome dysbiosis across early Parkinson’s disease, REM sleep behavior disorder and their first-degree relatives - Nature Communications

. The taxonomy table was then collapsed at levels of genus, family and phylum, and the merged abundance table was used for downstream analysis.The data were assessed for normal distribution before performing statistical analysis by the Shapiro–Wilk test. The univariate analysis of categorical data would be performed by the Chi-square test or Fisher’s exact test, where applicable.

The correlations of significantly differed taxa and different stages of α-synucleinopathy were further validated by applying Microbiome multivariable associations with linear model . The model included family id as the random effect to incorporate variability in family clustering . Multiple comparisons were adjusted using Benjamini–Hochberg method.

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