Parallel sequencing of extrachromosomal circular DNAs and transcriptomes in single cancer cells - Nature Genetics

Cell culture

Human tumor cell lines were obtained from ATCC or were provided by J. J. Molenaar . The identity of all cell lines was verified by short tandem repeat genotyping ; absence ofspp. contamination was determined with a Lonza MycoAlert Detection System. Cell lines were cultured in Roswell Park Memorial Institute 1640 medium supplemented with 1% penicillin, streptomycin and 10% FCS. To assess the number of viable cells, cells were trypsinized , resuspended in medium and sedimented at 500 g for 5 min.

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Gut microbiome dysbiosis across early Parkinson’s disease, REM sleep behavior disorder and their first-degree relatives - Nature CommunicationsMicrobiota-gut-brain axis may play an important role in Parkinson’s disease (PD). Here, the authors assess gut microbiota in early PD, REM sleep behaviour disorder (RBD) and first-degree relatives of RBD and show PD-like gut dysbiosis occurs in RBD and their first-degree relatives.
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