Methylmalonic acid promotes colorectal cancer progression via activation of Wnt/β-catenin pathway mediated epithelial–mesenchymal transition - Cancer Cell International

Human CRC cells HCT116 and SW480 were purchased from Cell Bank of Chinese Academy of Sciences in Shanghai. HCT116 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin . SW480 cells were cultured in L15 medium supplemented with 10% FBS and 1% penicillin–streptomycin.

For EDU assay, cells were treated with 50 μM EDU medium at 37 ℃ for 2 h, then fixed with 4% paraformaldehyde for 30 min. After permeabilized with 0.5% Triton-X, the cells were successively reacted with 1 × Apollo dyeing reaction solution and Hoechst 33,342 for 30 min. Finally, images were visualized using fluorescence microscope and analyzed by Image J and GraphPad Prism 6 software.

Health Health Latest News, Health Health Headlines

Similar News:You can also read news stories similar to this one that we have collected from other news sources.

NI cancer warriors taking on Coast to Coast challenge from Down to DonegalBoth men have undergone treatment for cancer and supported each other throughout.
Source: BelfastLive - 🏆 16. / 77 Read more »

Belfast mum given months to live after celebrating all-clear news with her kidsSometimes there are just no words... 💔
Source: BelfastLive - 🏆 16. / 77 Read more »