Effects of age, seasonality, and reproductive status on the gut microbiome of Southern White Rhinoceros (Ceratotherium simum simum) at the North Carolina zoo - Animal Microbiome

), and alfalfa hay were all offered in rotation for training and enrichment; these supplementary feeds were offered at 2 °C. Their outdoor holding comprised three adjoining paddocks totaling 1450 m², including a heated lean-to in the largest paddock.

We extracted Microbial DNA from the feces using the PowerFecal Pro DNA kit after two weeks of storage.

]. We eluted extracted DNA in 100 µl of the elution buffer and stored the DNA in elution tubes at -20 °C until the end of the sampling season.The Genomic Sciences Laboratory at North Carolina State University sequenced the extracted DNA using established methods for the hypervariable V3 and V4 regions of the 16S rRNA gene [

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