]. In the future, the findings presented here may be exploited for the development of therapeutic approaches that aim at restoring the tumor suppressive function of the p53/miR-34 pathway.HCT116, RKO, SW48, CCD-18Co, and SW620-Luc2 cell lines were cultured in McCoy’s 5 A medium with 10% fetal bovine serum containing 100 units/ml penicillin and 0.1 mg/ml streptomycin.
Total cellular RNA was isolated and purified from CRC cell lines according to manufacturer’s instruction . 1ug of total RNA was used for reverse transcription with Verso cDNA Synthesis Kit . Quantitative real-time polymerase chain reaction was performed using Fast SYBR Green Master Mix on a LightCycler 480 system. qRT-PCR data was normalized to
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