HER2 chimeric antigen receptor T cell immunotherapy is an effective treatment for diffuse intrinsic pontine glioma

were a kind gift from Dr. Michelle Monje and verified as Mycoplasma negative by PCR analysis at the WEHI internal facility. DIPG PDX cells were transduced and selected to express GFP-Firefly Luciferase for-A medium supplemented with 10 mM Hepes , 1 mM sodium pyruvate , 0.

. Antibodies used include anti-MYCTag-AF488 , anti-CD25-APC , anti-CD69-BV650 , anti-CD4-FITC , and anti-CD8-PE antibodies .Adherent target cells were seeded in triplicate in 96 flat bottom plates before co-incubation with effector CAR T cells at a range of effector:target ratios, and normalized for CAR expression. Cells were co-cultured in media containing 200 µM Propidium Iodide , and plates imaged using an IncyCyte every hour . Percentage lysis was determined as / × 100).

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