HeLa and HCT-116 cells were obtained from RIKEN BioResource Research Center . Cells were grown in alpha-modified minimum essential medium supplemented with 10% fetal bovine serum , 100 µg/mL streptomycin, and 100 units/mL penicillin. Cells were cultured under standard conditions at 37 °C in a humidified incubator containing 5% CO. Transfection of HeLa cells with the EGFP-TOP2B expression plasmid was performed using a FuGENE6 reagent according to the manufacturer’s instructions.
In brief, an FV1200-IX83 laser scanning confocal microscope with an oil-immersed 60× objective was used for fluorescence microscopy. For live cell imaging, cells grown on a glass-bottomed dish were placed on a stage top incubator that maintained a humidified atmosphere and 5% CO. Briefly, HeLa cells in a glass bottom dish were transfected with EGFP-TOP2B plasmid DNA. At 48 h after transfection, live cell imaging was performed as described above.
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