Peripheral blood mononuclear cells were isolated using Ficoll Paque Plus . Immune cell isolations were performed using specific kits from StemCell EasySep Kits for the isolation of CD14 as per manufacturer procedures. CD14+ monocytes were cultured at 37 °C and 5% COin RPMI medium supplemented with 10% fetal calf serum and 50 ng/mL macrophage colony-stimulating factor or granulocyte macrophage colony-stimulating factor for 7 days, replacing media and removing non-adherent cells at day 4.
In another experiment, MDM cells were treated with LPS , IFN-γ, IL-4 , or IL-1β for 24 h and MBOAT7 mRNA expression was assessed by PCR and normalized to untreated MDMs and GAPDH.Total RNA was extracted from cells using the Tissue Total RNA purification Mini Kit and Qiagen miRNeasy Mini Kit according to the manufacturer’s instructions. cDNA was reverse transcribed from total RNA using qScript® cDNA SuperMix according to the manufacturer’s instructions.
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Factors associated with COVID-19-related death using OpenSAFELY - NatureOpenSAFELY, a new health analytics platform that includes data from over 17 million adult NHS patients in England, is used to examine factors associated with COVID-19-related death. HKUniversity HKUniversity No shit? *puff puff puff puff
Source: medical_xpress - 🏆 101. / 51 Read more »
Brain fog of post-COVID-19 condition and Chronic Fatigue Syndrome, same medical disorder? - Journal of Translational MedicineBackground Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is characterized by persistent physical and mental fatigue. The post-COVID-19 condition patients refer physical fatigue and cognitive impairment sequelae. Given the similarity between both conditions, could it be the same pathology with a different precipitating factor? Objective To describe the cognitive impairment, neuropsychiatric symptoms, and general symptomatology in both groups, to find out if it is the same pathology. As well as verify if the affectation of smell is related to cognitive deterioration in patients with post-COVID-19 condition. Methods The sample included 42 ME/CFS and 73 post-COVID-19 condition patients. Fatigue, sleep quality, anxiety and depressive symptoms, the frequency and severity of different symptoms, olfactory function and a wide range of cognitive domains were evaluated. Results Both syndromes are characterized by excessive physical fatigue, sleep problems and myalgia. Sustained attention and processing speed were impaired in 83.3% and 52.4% of ME/CFS patients while in post-COVID-19 condition were impaired in 56.2% and 41.4% of patients, respectively. Statistically significant differences were found in sustained attention and visuospatial ability, being the ME/CFS group who presented the worst performance. Physical problems and mood issues were the main variables correlating with cognitive performance in post-COVID-19 patients, while in ME/CFS it was anxiety symptoms and physical fatigue. Conclusions The symptomatology and cognitive patterns were similar in both groups, with greater impairment in ME/CFS. This disease is characterized by greater physical and neuropsychiatric problems compared to post-COVID-19 condition. Likewise, we also propose the relevance of prolonged hyposmia as a possible marker of cognitive deterioration in patients with post-COVID-19.
Source: BioMedCentral - 🏆 22. / 71 Read more »
Cancer drug trial using COVID vaccine mRNA technology shows 'highly encouraging' resultsScientists are optimistic that mRNA vaccines will improve survival rates in many cancers that are currently hard to beat. A demonstrably harmful technology that couldn't make it out of animal trials over a 20 year period (without killing said animals) and now clearly harmful to humans in the couldn't be more massive or more conclusively bad Covid 'trial'. Snake oil, anyone? Shouldn’t be allowed, it’s changed the genetics of the human race! That’s a no from me
Source: SkyNews - 🏆 35. / 67 Read more »