By Neha MathurNov 21 2022Reviewed by Aimee Molineux In a recent study published in Emerging Infectious Diseases, researchers demonstrated the efficacy of two World Health Organization ‒recommended alcohol-based hand rub solutions to inactivate the monkeypox virus .
A concerning fact about MPXV that makes it a public health concern is that it is spreading among persons who have not traveled to disease-endemic areas. Its clinical and epidemiologic patterns are novel, unlike prior outbreaks. Also, it is remarkably more stable than other pox viruses. It is thus necessary to confirm which disinfectants and biocidal agents can effectively inactivate MPXV.
First, the team cultured Vero 76 cells in Dulbecco modified Eagle medium and seeded them at a concentration of 0.33 × 106 cells/mL for MPXV preparation. Next, following a change of medium, they inoculated these cells with MPXV at a multiplicity of infection of 0.01. The team incubated Vero 76 cells at 37°C until they observed a visible cytopathic effect .
They performed an endpoint dilution assay on Vero 76 cells and evaluated CPE microscopically after seven days. Next, they computed the 50% tissue culture infectious dose per milliliter for all four disinfectants at final concentrations of 20%, 30%, 40%, 60%. and 80%. Finally, the researchers used a suspension test to examine the virucidal activity of the WHO formulations I and II against MPXV.
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