T cells were added. Brefeldin A was added 5 h before the T cells were harvested at the 24-h and 48-h time-points for flow cytometric analysis of the cytokine and exhaustion profile. All flow cytometry data analysis was performed with FlowJo software. Antibodies used and their corresponding dilutions are listed in Supplementary TableCytotoxic effects were analyzed using 2D CellTox Green Cytotoxicity Assays according to the manufacturer’s protocol. Briefly, 5000 HBV-producing HepG2.2.
For each time-point, the following respiratory parameters were recorded in pmol per minute: basal OCR, extracellular acidification rate , ATP production, maximal respiratory capacity, spare respiratory capacity, glycolysis glycolytic capacity, and glycolytic reserve. Data [mean value ± standard error of the mean ] were analyzed using the Seahorse Wave software.T cells were prepared and treated as described above and then plated in different wells for collection every 24 h up to day 5.