). For analysis, either littermate controls were used or a corresponding control line from the initial Fgeneration was generated. The age of animals is given in the respective figure legend. All animals were maintained on a C57BL/6 background. Genotyping was performed on clips derived from earmarking according to standard protocols . The genotype was confirmed by regenotyping on a tail biopsy following euthanasia of the animal at the end of the respective experiment..
For statistical analysis of the behavioural data, we report the results of several different type 3 analysis of variance tests that calculated the main effects for the 5×FAD mice and myelin mutant mice as well as their interaction. All analyses were conducted in R . The ANOVA tests were computed using the afex package Tissue preparationasphyxiation and subsequently transcardially perfused with ice-cold Hank’s buffered salt solution and 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
. In brief, tissue samples were dehydrated with an ascending concentration of methanol in PBS . Tissue samples were then bleached with a 1:1:4 ratio of H:DMSO:methanol overnight at 4 °C. Further dehydration was followed by 100% methanol incubation at 4 °C , −20 °C and overnight storage at 4 °C. Samples were incubated the following day in methanol with 20% DMSO before subjecting them to gradual rehydration in a descending methanol in PBS series .
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