The gut microbiota in multiple sclerosis varies with disease activity - Genome Medicine

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The gut microbiota in multiple sclerosis varies with disease activity, finds a study published in GenomeMedicine.

] and individuals phenotyped at Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen . The CMBR cohort was recruited from urban areas in Denmark by advertisement in local newspapers, social media, and other online resources from November 2013 to November 2014.

Blood was drawn in the morning after an overnight fast from a cubital vein into an EDTA tube, centrifuged to separate plasma and cells, and immediately stored at −80°C until analysis. Collected plasma samples were further used for metabolic markers and cytokine measurement . Stools were collected according to International Human Microbiome Standards guidelines in kits by multiple sclerosis cases and HC at home and immediately stored at −20 °C until they were transported on dry ice and frozen 4–24 h later at −80°C in plastic tubes at the biobanks of Novo Nordisk Foundation Center for Metabolic Research or Glostrup Hospital. Stools were further subjected to shotgun sequencing , bacterial cell counting , and fecal water estimation .

 

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