Cells derived from 18 of the patients included in our study were screened for sensitivity to 349 drugs using DSRTThe 349 compounds from the anti-cancer Selleck Chemical library were pre-aliquoted at five concentrations, increasing in 10-fold steps from 1 nM to 10 μM, in 384-well plates using an Echo Liquid Handler. Each plate also contained eight positives and eight negative controls.
All patient samples were normalized to the median of the positive and negative controls per plate and presented as selective Drug Sensitivity Score , calculated using the online tool Breeze .PB samples at pre-treatment, 4 and 24 h after the start of treatment for proteomics, RNA sequencing, and targeted DNA-seq were sampled in BC Vacutainer CPTMononuclear cell preparation tubes with sodium heparin. Mononuclear cells were prepared by density gradient separation .
UiB NatureComms 🆘 Cure of cancer “If it is not possible here, the best thing is another country do because it benefits people, it’s not by flag. Humanity needs of someone who does something to heal the their evils.” Gilberto Chierice, researcher usponline cienciausp