, was used to assess in vitro sensitivity to VRC01 of HIV-1 Env-pseudotyped viruses . Full details on pseudovirus stock preparation, the TZM-bl assay and calculation of serum neutralization titers are reported in the appendix of ref.Using RNA samples from AMP participants who had acquired HIV-1 infection, the genomic region of the acquired HIV-1 isolate coding for the complete Env glycoprotein was sequenced and ancassette plasmid was synthesized by Genewiz.
Additional assays were performed on the VRC01 drug product . Neutralization titers are expressed as the concentration of the VRC01 drug product at which RLU were reduced by either 50% or 80% relative to virus control wells after subtraction of background RLU in cell control wells. The VRC01 drug product was assayed against each HIV-1 Env-pseudotyped virus three times, at starting concentrations of 100 and 5 μg ml, using eight three-fold serial dilutions. HIV-1 PVO.
HelpEndHIV If you can’t get diagnosed with hiv, you will never end hiv. Too many spreaders unfortunately.
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