Time-lapse live imaging of LGR5-tdTomato/p27-mVenus/Fucci-iRFP CCO20 organoids in a routine culture condition . 4D images were acquired with a 1-hour interval and max intensity projection images were converted to a 15 frames per second mov file. The quantification data is shown in Fig. 2j.Time-lapse live imaging of LGR5-tdTomato/p27-mVenus/Fucci-iRFP CCO32 organoids with or without camptothecin treatment .
4D images were acquired with a 1-hour interval, and max-intensity projection images were converted to a 15-fps movie. The quantification data is shown in Extended Data Fig. 4g.Time-lapse live imaging of LGR5-tdTomato/p27-mVenus/Fucci-iRFP CCO32 organoids with or without camptothecin and FAK inhibitor treatment. 4D images were acquired with a 1-hour interval and max-intensity projection images were converted to a 15-fps movie. The signals of LGR5-tdTomato are p27-mVenus are shown.